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Reagent Final Conc. Volume Mass Salmon Testes DNA (Sigma D-9156),
10.4 mg/ml, boil 5 min. before use optional: dextrane sulfate dH2O ad 20 ml (1 hour)Isolate 1-10 µg target cDNA from vector
Important: Extract the DNA within one week of receiving samples. Samples that have been processed should be frozen to prevent degradation. Freeze samples between use each day.
1. Add 600 ml of 50
